Single-Molecule Twist and Stretch of Mononucleosome using Optical Torque Wrench
نویسندگان
چکیده
منابع مشابه
Calibration of the optical torque wrench.
The optical torque wrench is a laser trapping technique that expands the capability of standard optical tweezers to torque manipulation and measurement, using the laser linear polarization to orient tailored microscopic birefringent particles. The ability to measure torque of the order of kBT (∼4 pN nm) is especially important in the study of biophysical systems at the molecular and cellular le...
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The recent advent of angular optical trapping techniques has allowed for rotational control and direct torque measurement on biological substrates. Here we present a method that increases the versatility and flexibility of these techniques. We demonstrate that a single beam with a rapidly rotating linear polarization can be utilized to apply a constant controllable torque to a trapped particle ...
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aAssociate Professor, Department of Clinical Sciences, School of Dental Medicine, University of Nevada Las Vegas. bProfessor, Restorative and Prosthetic Dentistry, College of Dentistry, The Ohio State University, Columbus, Ohio. (J Prosthet Dent 2012;108:401-403) 1 Instrument kit with available inserts, from left to right: 0.050in. hexagon straight (Zimmer part # HX1.25D), 0.048-in. 24 mmL hex ...
متن کاملOptical torque wrench: angular trapping, rotation, and torque detection of quartz microparticles.
We describe an apparatus that can measure the instantaneous angular displacement and torque applied to a quartz particle which is angularly trapped. Torque is measured by detecting the change in angular momentum of the transmitted trap beam. The rotational Brownian motion of the trapped particle and its power spectral density are used to determine the angular trap stiffness. The apparatus featu...
متن کاملdevelopment of different optical methods for determination of glucose using cadmium telluride quantum dots and silver nanoparticles
a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.2639